Description
Purpose of Use
Nucleic acid (DNA/RNA) extraction from microorganisms
(This kit was developed in order to easily extract and purify nucleic acids of pathogen, etc. included in various samples from them
as a prior step of gene amplification. Especially, it has excellence performance for trace amount of specimens.)
Features
- The Zirconia beads, which is twice hevier the specific gravity than glass beads,　can phisically disrupt impregnable　cell wall or endospore of Gram-positive anaerobic by shaking.
- DNA/RNA can be collected without digradation under disruption with guanidium.
Composition
- Beads filling tube　50 tubes
- Lysis buffer　10mL
- Phenol mixture　20mL
- SDS solution　10mL
- RNase free Distilled water　10mL
- 2mL tubes for　experiment　50 tubes
- Paper stand for ecperiment　1 pc
- Manual　1 pc
| MORA-EXTRACT Nucleic Acid Yield Comparison (Beads vs No beads) DNA/RNA Extraction Efficiency |
|||||||
|---|---|---|---|---|---|---|---|
| Organism | Cat. No. | Lysis + Beads | Lysis Only | Nucleic Acid Yield Ratio (beads/no beads) | |||
| Nucleic Acid Purity 260/280 |
Nucleic Acid |
Nucleic Acid Purity 260/280 |
Nucleic Acid Concentration μg/mL |
||||
| Gram Negative Bacteria |
Aeromonas hydrophilia | GTC 140 | 1.74 | 96.8 | 1.48 | 51.2 | 1.89 |
| Escherichia coli | GTC 1061 | 1.85 | 117.4 | 1.46 | 64.4 | 1.82 | |
| Salmonella enterica | GTC 133 | 1.84 | 104.3 | 1.38 | 76.5 | 1.36 | |
| Gram Positive Bacteria |
Staphylococcus aureus | GTC 1186 | 1.8 | 94.3 | 1.32 | 48.4 | 1.95 |
| Listeria monocytogenes | GTC 149 | 1.8 | 97.2 | 1.45 | 33.8 | 2.88 | |
| Bacillus cereus | GIFU 12028 | 1.76 | 76.1 | 1.45 | 32.6 | 2.33 | |
| Clostridium botulinum A | GAI 92003 | 1.84 | 68.4 | 1.45 | 20.5 | 3.34 | |
| Clostridium botulinum E | GAI 92012 | 1.77 | 91.5 | 1.5 | 24.7 | 3.70 | |
| Fungi | Candida albicans | GTC 1754 | 1.78 | 51.4 | 1.6 | 28.3 | 1.82 |
