Description
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The kit is designed to determine cellular viability
Background: The mitochondrial activity of the cells is reflected by the conversion of the tetrazolium salt MTT into formazan crystals, which can be solubilized for homogenous measurement. Thus any increase or decrease in viable cell number can be detected by measuring formazan concentration spectrophotometrically using a plate reader at 570 nm vs 690 nm. In this assay, the resulting formazan crystals are directly redissolved without removing the culture medium. This kit is suitable for cells in suspension and faster, but less sensitive, than MTT Cell Proliferation Assay A.
