MuRF1 [untagged]

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Species Reactivity: human  

Source: E.coli  

Formula: 50mM sodium Bicarbonate pH10, 250mM NaCl, 0.03% Brij35, 0.5mMTCEP  

Concentration: 0.25mg/ml  

Molecular Weight: ~40.2kDa  

Protein Sequence: Accession number: NP_115977.2 For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E3 Ligase Assay: The ubiquitin conjugating activity of MuRF1 was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme His-UBE1, the E2 conjugating enzyme His-UBE2D3 (UbcH5c) (several E2s were tested, data generated with this E2 is provided by way of example) and ubiquitin. Incubation of MuRF1 for 60 minutes at 30oC in the presence of ubiquitin, His-UBE1, His-UBE2D3 and ATP (Lane 1) was compared alongside two control reactions with either ATP (Lane 2) or MuRF1 (Lane 3) excluded from the reaction. Ubiquitin conjugates were identified by Western blotting using an anti-ubiquitin conjugate antibody and these were observed only in the presence of both ATP and MuRF1.