PAP Pen Super-Liquid Blocker

PAP Pen Super-Liquid Blocker is a ready-to-use hydrophobic barrier pen, designed for immunohistochemistry applications. A minimal amount of antibody is constrained by surface tension within a user-defined border drawn with the pen around a tissue section. The pen is stable up to 129°C in microwave heat.

This ready-to-use hydrophobic barrier pen is designed for immunohistochemistry applications. The surface tension provided by the circle, square, or any other shapes drawn to fit tissue sections with the pen, ensures that only the amount of antibody solution needed for the sufficient reaction will be applied. The pen is stable up to 129 degrees C in microwave heat.

The PAP Pen is an effective tool for immunostaining procedures by the Peroxidase-Antiperoxidase (PAP) method, ABC method, LSAB method, polymeric method, immunofluorescence method, ASD method, Enzyme method and Frozen Section method.

For paraffin sections, the PAP Pen should be applied after deparaffinization. For frozen sections, the PAP Pen can be applied after fixation.

Specifications


• Liquid volume: 6 ml

• Size: 141 mm (L) x 15 mm (D)

• Pen tip: 4 mm


Directions


• Shake well before use. Push down on the tip a couple times to release liquid into the tip.

• Deparaffinize tissue sections and hydrate to water. Perform antigen retrieval if necessary.

• Wipe away excess liquid around the section on slide with Kimwipes or tissue paper.

• Allow tip to get moderately wet. Encircle the tissue sections or draw lines on both sides of the section and let dry for 10 to 15 seconds.

• Apply drops of washing buffer to cover the section.

• Proceed with standard immunohistochemistry procedure.