Description
For cloning, DNA expression and site-directed mutagenesis _III_Pyrococcus furiosus_IIII_ DNA polymerase (Pfu DNA polymerase) gene was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3' -> 5' exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.
* Pfu DNA polymerase is thermostabe and has low error rates.
* It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
* Pfu DNA polymerase-generatedPCR fragments are blunt-ended.
Quality Assurance: Greater than 95% of protein determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.
PCR Test: Good amplification result was obtained in PCR reaction using lambda DNA as a template.
Storage: Store at -20ðC
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* Pfu DNA polymerase is thermostabe and has low error rates.
* It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
* Pfu DNA polymerase-generatedPCR fragments are blunt-ended.
Quality Assurance: Greater than 95% of protein determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.
PCR Test: Good amplification result was obtained in PCR reaction using lambda DNA as a template.
Storage: Store at -20ðC
