Description
| Gene/Insert name | NAD(P)H-flavin oxidoreductase of Vibrio fischeri (FRase) |
|---|---|
| Vector backbone | pUC8 |
| Vector type | E. coli |
| Backbone size w/o insert (bp) | 2,650 |
| Bacterial resistance | Ampicillin |
| Growth strain | JM83 |
| High or low copy | High copy |
| Size | 10ug |
Gene coding region (ORF: Clytin II)
Nucleotide sequence
ATTCCCGACATCATAAGTTGTGCAGACAAGAATGTCTGTGGATTAAAATTTCACAAGTAAGGTTTATTAT TATGACGCATCCAATTATTCATGATCTTGAAAATCGTTATACATCAAAAAAATATGACCCATCAAAGAAA GTATCTCAAGAAGATTTAGCGGTTTTGCTTGAGGCTCTGCGTTTATCTGCTTCTTCAATTAATTCACAGC CTTGGAAATTCATTGTTATTGAATCCGATGCAGCGAAGCAACGTATGCATGATTCGTTTGCAAATATGCA TCAGTTTAATCAACCTCACATCAAAGCGTGTTCTCATGTGATTTTATTTGCAAATAAGCTTTCGTATACA CGAGATGATTATGATGTGGTTTTATCTAAAGCGGTTGCTGACAAGCGTATTACTGAAGAGCAAAAAGAAG CTGCTTTTGCTTCGTTTAAGTTTGTAGAATTGAACTGTGATGAAAATGGTGAGCATAAAGCATGGACTAA GCCTCAAGCTTATTTAGCTCTTGGTAATGCTCTGCATACATTAGCTAGACTGAACATTGACTCAACAACA ATGGAAGGCATTGATCCTGAATTATTGAGTGAAATTTTTGCTGATGAATTAAAAGGGTATGAATGTCATG TTGCTTTAGCCATTGGTTATCATCATCCAAGCGAAGATTATAATGCCTCTTTGCCTAAGTCTCGTAAGGC ATTTGAAGACGTAATTACCATCCTTTAGATTCTTAATGTTTGAGATGAAGAAAAGCCAGCGATTTAGCTG GCTTTTGTTTGTGCAAAATGTTCCTAATGGCGTATTACTACGGTAGGAAGTCTATTTAAAGTTTCTTTTA CTCTTTGGTAATTATTGTCAATTACGCGGAAATCATTATCTAAGTAGGTCGAC
Amino acid sequence
MTHPIIHDLENRYTSKKYDPSKKVSQEDLAVLLEALRLSASSINSQPWKFIVIESDAAKQRMHDSFANMH QFNQPHIKACSHVILFANKLSYTRDDYDVVLSKAVADKRITEEQKEAAFASFKFVELNCDENGEHKAWTK PQAYLALGNALHTLARLNIDSTTMEGIDPELLSEIFADELKGYECHVALAIGYHHPSEDYNASLPKSRKA FEDVITIL*
