Description
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The kit is designed for the rapid determination of polyphenols in various samples.
Background: Phenolic Quantification Assay is based on Folin-Ciocalteu method. The FC reagent contains phosphomolybdic/ phosphotungstic acid complexes The method relies on the transfer of electrons in alkaline medium from phenolic compounds to form a blue chromophore constituted by a phosphotungstic/ phosphomolybdenum complex where the maximum absorption depends on the concentration of phenolic compounds. The reduced Folin-Ciocalteu reagent is detectable with a spectrophotometer in the range of 690 to 710 nm. The reaction temperature has been used to reduce the time necessary to attain the maximum color (T= 37°C). Generally, gallic acid is used as the reference standard compoundand results are expressed as gallic acid equivalents (mg/ml). The F-C assay has been used as a measure of total phenolics in natural products, but the basic mechanism is an oxidation/reduction reaction. In the original F-C assay, the carbonate buffer is used for pH adjustment and the end-point of the reaction was attained after 120 min at room temperature, which makes its implementation for routine analysis difficult.
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