PORCINE CIRCOVIRUS 2 CAPSID PROTEIN (E. COLI), HIS-TAG

Porcine circovirus 2 (PCV-2) capsid antigen is a recombinant protein, purified from E. coli with a C-terminal His-tag, to greater than 90% purity.

PRODUCT DETAILS – PORCINE CIRCOVIRUS 2 CAPSID PROTEIN (E. COLI), HIS-TAG

• C-terminal 6xHis tagged Capsid (PCV2) protein (a.a. 1-234) (GenBank Accession No. AFC95829).
• Recombinant protein purified from E.coli.
• Protein in PBS with 8M Urea.
• Purity >90%, based on SDS PAGE.

BACKGROUND

Porcine circovirus type 2 (PCV2) belongs to the viral family Circoviridae and to the genus Circovirus. Circoviruses are small, single-stranded nonenveloped DNA viruses that have an unsegmented circular genome. PCV2 is the primary causative agent of several syndromes collectively known as porcine circovirus-associated disease (PCVAD). It was discovered in association with postweaning multisystemic wasting syndrome (PMWS) in Canadian weaning piglets in 1991. PCV2 infection is now present in every major swine-producing country in the world (Gillespie et al., 2009).

The attachment of PCV2 to target cells occurs through chondroitin sulfate B and probably other receptors. Internalisation is not fully known likely involves a specific receptor and the GAGs may mediate internalisation and binding to the target cells. Much of the time the infection is subclinical but in some circumstances such as coinfections with other respiratory pathogens it can cause the Post-weaning Multisystemic Wasting Syndrome (PMWS), clinically characterized by wasting respiratory disease, and enteritis (Saade et al., 2020). PCV2 is a member of the Porcine Respiratory Disease Complex (PRDC) alongside viruses such as swine Influenza A Virus (swIAV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and bacteria like Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae and Bordetella bronchiseptica (Saade et al., 2020). Where multiple infectious agents are involved in the development of a clinical condition the treatment can be much more challenging and the outcome less certain.

REFERENCES

• Gillespie J, Opriessnig T, Meng XJ, Pelzer K, Buechner-Maxwell V. Porcine circovirus type 2 and porcine circovirus-associated disease. J Vet Intern Med. 2009 Nov-Dec;23(6):1151-63.
• Saade G, Deblanc C, Bougon J, Marois-Créhan C, Fablet C, Auray G, Belloc C, Leblanc-Maridor M, Gagnon CA, Zhu J, Gottschalk M, Summerfield A, Simon G, Bertho N, Meurens F. Coinfections and their molecular consequences in the porcine respiratory tract. Vet Res. 2020 Jun 16;51(1):80.