proAKAP4 4MID Kit (ELISA, Dog)

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The proAKAP4 ELISA kit allows you to quantify proAKAP4 in samples of dog semen as a biomarker of sperm quality.

Innovative complete immunoassay to assess semen quality in dog

The proAKAP4 ELISA kit is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of dog semen. This ELISA Kit is the 1st functional test to evaluate sperm quality.

The dosage of the proAKAP4 biomarker with the ELISA Kit, will bring you:


• Objective information on semen quality

• Qualification of each breeding male and their ejaculates

• Pertinent parameter to improve sperm cryopreservation

• A functional indicator correlated to fertility index


The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.

Technical data


• 40 samples can be run in duplicate

• Working with fresh ejaculate or with frozen semen 

• Easy to use immunoassay / Manual and automated assay

• Robust and reproductible method


Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Reagents provided: The complete proAKAP4 ELISA kit contains all reagents and buffers required for the quantification of proAKAP4 protein in dog semen.


• One Microplate pre-coated with the Capture Antibody

• One vial of Standard

• One vial of Detection Antibody

• One bottle of the Enzyme Reagent

• One bottle of 10X Wash Buffer

• One bottle of the Sample Diluant

• One bottle of the Acid Stop Solution

• Three ELISA plate sealers