Description
Explore all PUREfrex® products and accessories from our PUREfrex® homepage.
PUREfrex® 2.0 reconstituted coupled transcription/translation system is a new and improved version of PUREfrex® 1.0 that debuted in 2011. By improving the purification process for components of PUREfrex® 2.0RNase, β-galactosidase and LPS contamination has been reduced, the latter to around 0.1EU/µL of reaction mixture. This, together with a newly optimized composition has boosted protein yield 2-10 times compared to PUREfrex® 1.0. Further, all proteinaceous components of PUREfrex® 2.0 are free of fusion tags, allowing users the freedom to incorporate any chosen tag for protein purification/detection.
For proteins whose functional conformation requires the assistance of molecular chaperones or the creation of disulfide bonds, we offer supplemental reagents that work in conjunction with PUREfrex® 2.0.
Supplements – Molecular Chaperones
These are supplements for PUREfrex® series reconstituted coupled transcription/translation systems to promote production and solubility of aggregation-prone proteins.
Supplements – Forming disulfide bonds
These are supplements for PUREfrex® series reconstituted coupled transcription/translation systems to promote synthesis of functional proteins containing disulfide bonds.
