Description
Applications: Studies on homologous recombination in mammals including human
Studies on the interaction of Rad51 protein with various proteins
To be used as a standard for Western blotting Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNAstrands (1). Rad51 functionally and physically interacts with its paralogs Dmc1 Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1 BRCA2, and P53 forthemaintenance of genome stability (1).
Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein (Fig. 1). Since the tag was removed from the recombinant protein (it still containsGly-Ser-His at the N-terminal), it has been shown to retain nuclear filament Forming and strand-exchange activity as well as interaction with Rad52 (2). This product was confirmed to possess single strand DNA stimulated ATPase activity.
Data Link: UniProtKB/Swiss Prot Q06609 (RAD51_HUMAN)
Storage: Store at -70°C
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Studies on the interaction of Rad51 protein with various proteins
To be used as a standard for Western blotting Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNAstrands (1). Rad51 functionally and physically interacts with its paralogs Dmc1 Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1 BRCA2, and P53 forthemaintenance of genome stability (1).
Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein (Fig. 1). Since the tag was removed from the recombinant protein (it still containsGly-Ser-His at the N-terminal), it has been shown to retain nuclear filament Forming and strand-exchange activity as well as interaction with Rad52 (2). This product was confirmed to possess single strand DNA stimulated ATPase activity.
Data Link: UniProtKB/Swiss Prot Q06609 (RAD51_HUMAN)
Storage: Store at -70°C
