Description
S1 Nuclease is a single-stranded-specific endonuclease that hydrolyzes single-stranded DNA and RNA into acid-soluble 5’- mononucleotides. It hydrolyzes single-stranded regions in duplex DNA such as loops and gaps.
This S1 Nuclease is from Aspergillus sp.
SPECIFICATIONS:
- Storage: 30mM sodium acetate (pH4.6), 50mM NaCl, 1M ZnCl2, 50% glycerol
- Activity: ≥100 units/μl
- Molecular Weight: 32,000 glycoprotein containing Zn2+
- Optimum pH and temperature: See Figure
- pH and thermal stability: See Figure
ASSAY: One unit of enzyme hydrolyzes 1μg of heat-denatured calf thymus DNAinto acid-soluble form at 37°C, pH4.6 in one minute.
Composition of Supplied Reagents (Store at -20°C):
- 10×S1 Nuclease Buffer: 300mM Sodium acetate (pH4.6), 2800mM NaCl, 10mM ZnSO4
