SUMO1 [6His-tagged]

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Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~13.5 kDa  

Protein Sequence: Accession number: NP_003343. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E2 Thioester SUMO-1 Loading Assay:  The activity of His-SUMO-1 was validated by loading His-SUMO-1 onto the active cysteine of the UBE2I E2 enzyme via a transthiolation reaction. Incubation of His-SUMO-1, SAE1/SAE2 and UBE2I enzymes in the presence of ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the His-SUMO-1/UBE2I thioester bond to the reducing agent DTT was confirmed.