Thermo T7 RNA Polymerase ((TT7))

To create RNA proble of high indication. To create precursor for RNA splicing reaction. To create mRNA with Cap analog as a primer. To create mRNA for in vitro TranslationThermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50oC. The half-life of the enzyme at 50°C is approximately 85 min.

Features: Exhibits greater specific activity than WT-enzyme at 37-50°C.

Application: RNA probe preparation

RNA synthesis for in vitro translation

RNA synthesis for RNA splicing studies

Capped mRNA synthesis using a cap analogue