Description
For real time PCR THUNDERBIRD® SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except
ROX reference dye and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments. This product is an improved version of Realtime PCR Master Mix (Code No. TYB-QPK-201) and Realtime PCR Master Mix -Plus- (Code No. TYB-QPK-212). In particular, the reaction specificity and PCR efficiency is enhanced.
The SYBR® Green I assay system utilizes fluorescent emission when SYBR® Green is intercalated into double-stranded DNA. The signal depends on the amount of amplified DNA. However, this system cannot distinguish between target and non-specific amplicons. Therefore, melting curve analysis is necessary after amplification.
Features: -High specificity
The specificity for the detection of low-copy targets is improved.
-Homogeneous amplification
The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)
-Broad dynamic range
High specificity and effective amplification enable the detection of a broad dynamic range.
-Compatibility for various real-time cyclers.
The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit,the kit can be applied to real-time cyclers that require a passive reference dye.
-Hot start PCR
The master mix contains anti-Taq DNA polymerase antibodies for hot start technology.
The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.
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ROX reference dye and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments. This product is an improved version of Realtime PCR Master Mix (Code No. TYB-QPK-201) and Realtime PCR Master Mix -Plus- (Code No. TYB-QPK-212). In particular, the reaction specificity and PCR efficiency is enhanced.
The SYBR® Green I assay system utilizes fluorescent emission when SYBR® Green is intercalated into double-stranded DNA. The signal depends on the amount of amplified DNA. However, this system cannot distinguish between target and non-specific amplicons. Therefore, melting curve analysis is necessary after amplification.
Features: -High specificity
The specificity for the detection of low-copy targets is improved.
-Homogeneous amplification
The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)
-Broad dynamic range
High specificity and effective amplification enable the detection of a broad dynamic range.
-Compatibility for various real-time cyclers.
The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit,the kit can be applied to real-time cyclers that require a passive reference dye.
-Hot start PCR
The master mix contains anti-Taq DNA polymerase antibodies for hot start technology.
The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.
