Description
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Trolox is a water soluble analog of tocopherol (Vitamin E) which is a very strong antioxidant, commonly used to measure antioxidant capacity. Vitamin E is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables
RECOMMENDED USES
To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC). It could be used for biological samples.
COMPATIBLE BUFFERS
| Product | Reference |
| 150 mM Sterile PBS pH 7.4 |
STANDARD CALIBRATION
Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:
|
| Standard (µl) | Diluent (µl) | TEAC (µM) |
| 1 | 0 | 1000 | 0 |
| 2 | 50 | 950 | 50 |
| 3 | 100 | 900 | 100 |
| 4 | 200 | 800 | 200 |
| 5 | 400 | 600 | 400 |
| 6 | 600 | 400 | 600 |
| 7 | 800 | 200 | 800 |
| 8 | 1000 | 0 | 1000 |
*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.
e-BQC SPECIFICITY
TROLOX is more predominantly present in Q1 where it has lower detection limits and less interferences. It is recommended to use Q1 for calibrations at low concentrations of TROLOX.
|
| Q1 | Q2 | Qt |
| Sensitivity | 0.05 µC/ µM | 0.05 µC/ µM | 0.11 µC/ µM |
| LOD | 2 µM | 90 µM | 42 µM |
| LOQ | 7 µM | 300 µM | 140 µM |
| LOB | 0.8 µM | 7 µM | 8 µM |
| RSD | 15 % | 13 % | 14 % |
COMPARISON WITH TAC ASSAY KITS
If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an TROLOX standard could be used:
| Product | Reference |
| DMPD | |
| FRAP | |
| FAST FRAP | |
| ORAC |
