UBE1 [6His-tagged]

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Species Reactivity: Human  

Source: Sf21 insect cell-baculovirus expression  

Formula: 50 mM HEPES pH 7.5 (NaOH), 150 mM NaCl, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: ~121 kDa  

Protein Sequence: Accession number: NP_003325. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E1-Ubiquitin Thioester Loading Assay:  The activity of His-UBE1 was validated by loading ubiquitin onto the active cysteine of His-UBE1. Incubation of the His-UBE1 enzyme in the presence of ubiquitin and ATP at 30oC was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/His-UBE1 thioester bond to the reducing agent DTT was confirmed.