UBE2F (NCE2) [GST-tagged]

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Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~48 kDa  

Protein Sequence: Accession number: NP_542409. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  E2-NEDD8 Thioester Loading Assay: The activity of GST-UBE2F was validated by loading E1 APP-BP1/Uba3 activated NEDD8 onto the active cysteine of the GST-UBE2F E2 enzyme via a transthiolation reaction. Incubation of the APP-BP1/Uba3 and GST-UBE2F enzymes in the presence of NEDD8 and ATP at 30oC was compared at two time points, T0 and T10 minutes. The sensitivity of this NEDD8/GST-UBE2F thioester bond to the reducing agent DTT was confirmed.