UBE2I (Ubc9) [6His-tagged]

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Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~20 kDa  

Protein Sequence: Accession number: NP_003336. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  SUMO-E2 Thioester Loading Assay: The activity of His-UBE2I was validated by loading E1 SAE1/SAE2 activated SUMO onto the active cysteine of the His-UBE2I E2 enzyme via a transthiolation reaction. Incubation of the SAE1/SAE2 and His-UBE2I enzymes in the presence of SUMO and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the SUMO/His-UBE2I thioester bond to the reducing agent DTT was confirmed.