UBE2I (Ubc9) [GST-tagged]

Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.

Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~45 kDa  

Protein Sequence: Accession number: NP_003336. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  SUMO-E2 Thioester Loading Assay: The activity of GST-UBE2I was validated by loading E1 SAE1/SAE2 activated SUMO onto the active cysteine of the GST-UBE2I E2 enzyme via a transthiolation reaction. Incubation of the SAE1/SAE2 and GST-UBE2I enzymes in the presence of SUMO and ATP at 30 °C was compared at two time points, T0 and T10 minutes.  Sensitivity of the SUMO/GST-UBE2I thioester bond to the reducing agent DTT was confirmed.