UBE2M (Ubc12) [6His-tagged]

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Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~25 kDa  

Protein Sequence: Accession number: AAH58924. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  E2-NEDD8 Thioester Loading Assay: The activity of His-UBE2M was validated by loading E1 APP-BP1/Uba3 activated NEDD8 onto the active cysteine of the His-UBE2M E2 enzyme via a transthiolation reaction. Incubation of the APP-BP1/Uba3 and His-UBE2M enzymes in the presence of NEDD8 and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the NEDD8/His-UBE2M thioester bond to the reducing agent DTT was confirmed.