UBE2N (UBC13) [6His-tagged]

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Species Reactivity: Human  

Source: Sf21 insect cell-baculovirus expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~20 kDa  

Protein Sequence: Accession number: NP_003339. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  E2-Ubiquitin Thioester Loading Assay: The activity of His-UBE2N was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the His-UBE2N E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and His-UBE2N enzymes in the presence of ubiquitin and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/His-UBE2N thioester bond to the reducing agent DTT was confirmed.