UBE2W (Ubc16) [6His-tagged]

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Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~22.2 kDa  

Protein Sequence: Accession number: NP_001001481.1. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  E2-Ubiquitin Thioester Loading Assay: The activity of His-UBE2W was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the His-UBE2W E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and His-UBE2W enzymes in the presence of ubiquitin and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/His-UBE2W thioester bond to the reducing agent DTT was confirmed.