USP1 [6His-tagged]/UAF1 [untagged]

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Species Reactivity: human  

Source: Insect sf21  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: USP1 = 90.5 kDa/UAF1 = 76.2 kDa  

Protein Sequence: Accession number: USP1 = NP_003359 and UAF1 = NP_065890. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: Deubiquitylase Enzyme Assay: The activity of 6His-USP1/UAF1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of 6His-USP1/UAF1 was compared confirming the deubiquitylating activity of 6His-USP1/UAF1.