VECTASTAIN[R] Elite ABC-HRP Kit, Peroxidase (Mouse IgG)

Features:

• Advanced avidin/biotin technologyThe Elite [R]ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
• Highest sensitivity, low background. The VECTASTAIN[R] Elite [R]ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN[R] ABC Kit with the same low background.
• Cost effective: Higher sensitivity leads to lower cost per slide
• Available without ('Standard' kit) or with biotinylated species-specific or universal secondary antibodies
• Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Kit reagents.

• 2 ml Reagent A
• 2 ml Reagent B
• 1 ml biotinylated horse anti-mouse IgG
• 3 ml normal horse serum

Kit contains sufficient reagents to stain approximately 500-1000 sections.

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems, make the peroxidase enzyme a preferred choice in many applications. (e.g. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

Sufficient reagents to stain approximately 500-1000 sections.

The VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.

The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed.