Xantina Oxidasa activity assay kit

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The Oil Red O kit is used to demonstrate adipocites and neutral triglicerides. This is also useful to demonstrate adipogenesis. Fat cells and neutral fat will be coloured in red and the nuclei in blue.

Uses and sample preparation:
This kit can be used in frozen tissue sections, fresh smears or touch preps. It is recommended the use of saline formalin or Baker’s fixative in order to maintain the phospholipids

Components:


• Mayer’s hematoxylin

• Absolute propylene glicol

• 85% propylene glicol

• Oil Red O Reagent


Short procedure:


1. Rinse slides with distilled water

2. Place in absolute propylene glycol

3. Place in Oil Red O solution

4. Place in 85% propylene glycol

5. Rinse in two changes of distilled water

6. Place in Hematoxylin

7. Rinse well with water

8. Clear and coverslip


Staining characteristics:
Fat cells and neutral fat can be coloured in red and the nuclei in blue-purple.