Custom Baculo

 

Custom Services

Orbigen offers a wide range of custom services. Please contact our sales department for more information or to place an order.

Subcloning a Gene of Interest into a Baculovirus Transfer Vector
Read More

Generation, Identification and Purification of Recombinant Baculovirus
Read More

Plaque purification of recombinant Baculovirus supernatant and isolation of individual Baculovirus clones
Read More

Amplification of Recombinant Baculovirus
Read more

Large-Scale Expression of Proteins from Recombinant Baculovirus
Read More

Maxi-Prep and Purification of High-Copy Number Plasmids (5-10 mg)
Read More

Subcloning a Gene of Interest into a Baculovirus Transfer Vector

The service includes:
Isolating the gene of interest from a customer-supplied vector and subcloning it into a baculovirus transfer vector.
Verification by restriction mapping that the subcloned insert is inserted correctly.
Medium-scale preparation of the recombinant Baculovirus transfer plasmid.
Purification of the recombinant plasmid to be suited for construction of recombinant virus.
What the customer has to provide: Gene of interest cloned in any vector.

What the customer will receive: 20 µg of ready-to-use recombinant Baculovirus transfer plasmid containing the gene of interest.


Price: $2,000
Turn-around time: 2 weeks
Generation, Identification and Purification of Recombinant Baculovirus

The service includes:
Cotransfection of recombinant Baculovirus transfer vector containing the gene of interest with linearized Baculovirus DNA.
Analysis of obtained recombinant Baculoviruses versus background control by end-point dilution.
Amplification of recombinant Baculovirus stock to a high-titer stock of over 1 x 10(8) virus particles per ml.
Titration of final high titer stock.
What the customer has to provide: 10 µg of recombinant Baculovirus transfer plasmid containing gene of interest.

What the customer will receive: 30 ml of high-titer recombinant Baculovirus stock solution ready to express the protein of interest.


Price: $2,000
Turn-around time: 4 weeks
Plaque purification of recombinant Baculovirus supernatant and isolation of individual Baculovirus clones

The service includes:
Plaque assay from recombinant Baculovirus stocks
Baculovirus isolation from 10 individual plaques
Amplification of the Baculovirus plaque picks to a high-titer stock.
What the customer has to provide: 1 ml of recombinant Baculovirus stock solution.

What the customer will receive: High-titer recombinant Baculovirus stock solution of 10 individual Baculovirus plaque-picks, 1 ml each.


Price: $1,000
Turn-around time: 4 weeks
Amplification of Recombinant Baculovirus

This service includes:
Low titer recombinant Baculovirus solution will be amplified to a titer of at least 1 x 10(8) virus particles per ml.
Titration of amplified Baculovirus solution by plaque assay.
What the customer has to provide: 1 ml of recombinant Baculovirus solution.

What the customer will receive: 500 ml of high-titer recombinant Baculovirus stock solution.


Price: $500
Turn-around time: 2 weeks
Large-Scale Expression of Proteins from Recombinant Baculovirus

This service includes:
10(9) insect cells will be infected with recombinant Baculovirus (MOI of 5).
Three days after infection, cells will be harvested, frozen and shipped to customer along with high-titer Baculovirus supernatant obtained from the infected insect cells.
What the customer has to provide: 30 ml of high-titer recombinant Baculovirus solution.

What the customer will receive: 10(9) infected insect cells expressing the protein of interest. The cells will be shipped on dry ice. Additionally, customer will receive 1 liter of high-titer recombinant Baculovirus solution.


Price: $1,000
Turn-around time: 2 weeks
Maxi-Prep and Purification of High-Copy Number Plasmids (5-10 mg)

This service includes:
Transfection of plasmid of interest into appropriate host bacteria.
Large-scale preparation of desired plasmid using 500 ml of LB-broth.
Purification of plasmid (maxi-prep) over DNA affinity matrix.
Quantification by UV and gel electrophoresis. High copy number plasmids will give a yield between 5 to 10 mg.
What the customer has to provide: 1 µg of desired plasmid.

What the customer will receive: 5 to 10 mg of purified plasmid.


Price: $500
Turn-around time: 1 week
back to top