Rat SPON2(Spondin 2) ELISA Kit
The activity loss rate (stability) of this kit is less than 5% within the expiration date under appropriate storage conditions. To minimize extra influence on the stability, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. When not in use, kit components should be refrigerated. All reagents should be brought to room temperature before use. It is recommended that all standards, controls, and samples be run in duplicate. Do not mix or interchange different reagent lots from various kit lots.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2 (SPON2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 2 (SPON2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 2 (SPON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spondin 2 (SPON2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
All kits are custom made on-demand. Please allow 10-15 working days for production and 2-3 days for shipment.
Marketed under license from USCN Life Science Inc.
Assay Length 4.5 h
Sample Type Serum, plasma and other biological fluids.
Detection Range 0.156-10ng/mL. The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
Specificity This assay has high sensitivity and excellent specificity for detection of Spondin 2 (SPON2). No significant cross-reactivity or interference between Spondin 2 (SPON2) and analogues was observed.
Inter-Assay Precision <12%
Intra-Assay Precision <10%
Shipping Conditions 2-8 °C
Storage Temperature -20 °C