MOUSE ANTI-MUMPS VIRUS NUCLEOPROTEIN (6007)

Mouse anti Mumps virus Nucleoprotein (6007) antibody is specific for Mumps virus Nucleoprotein and has been developed for use in ELISA and immunofluorescence.

PRODUCT DETAILS – MOUSE ANTI-MUMPS VIRUS NUCLEOPROTEIN (6007)

• Mouse anti Mumps virus Nucleoprotein (6007).
• Specific for the nucleoprotein of Mumps virus. Antibody does not cross-react with Measles virus, Respiratory Syncytial virus, Parainfluenza 1, 2 or 3 viruses
• Purified preparations consist of >90% pure mouse monoclonal antibody purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
• Presented in PBS pH7.2 with 0.1% sodium azide.
• For use in ELISA and immunofluorescence.
• Can be used with Goat anti mouse IgG HRP and PanBlock ELISA Blocking Buffer.

BACKGROUND

Mumps is a contagious viral illness and was once a common childhood disease. However, since the introduction of widespread vaccination, the incidence of Mumps in the population has significantly declined. The incubation period for Mumps is 15-24 days, with a median of 19 days before symptoms occur. The hallmark of Mumps infection is swelling of the parotid gland alongside headache, fever, fatigue, anorexia and malaise. The disease is usually self-limited with individuals experiencing a full recovery. Once an infection has run its course, a person is typically immune for life. In adult men and women aseptic meningitis and encephalitis are common complications of Mumps together with orchitis, oophoritis, deafness and pancreatitis. Mumps is vaccine-preventable and vaccination has been highly effective in reducing the occurrence of Mumps across the world (Hviid et al., 2008).

Mumps is caused by a negative-sense single-stranded RNA paramyxovirus which is transmitted by respiratory droplets or direct contact with an infected person. Humans are the only natural host for the virus. Virions are enveloped and contain fusion and attachment proteins on the virion surface. The nucleoprotein of the Mumps virus, also known as a nucleocapsid, is the basic architecture of the virus, comprised of a core of nucleic acid captured in a protein coat. Mumps virus encodes 9 proteins, three of which are required for replication of its genome; the nucleocapsid protein (N), phosphoprotein (P), and the large protein (L). Mumps nucleocapsid protein (N) encapsidates the genome, protecting it from nucleases and homomultimerizes to form the nucleocapsid. The nucleocapsid (NC) has a helical structure, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. Mumps hemagglutinin-neuraminidase (HN) is a major target for neutralizing antibody and NP is an immunodominant antigen (Latner, 2017).

Laboratory diagnosis of Mumps is currently based on isolation of virus, detection of viral nucleic acid, or serological confirmation of IgM Mumps antibodies.

REFERENCES

• Hviid et al. (2008). Mumps. Lancet. 2008 Mar 15;371(9616):932-44.
• Latner et al. (2017). Mumps Virus Nucleoprotein and Hemagglutinin-Specific Antibody Response Following a Third Dose of Measles Mumps Rubella Vaccine. Open Forum Infect Dis. 2017;4(4).