BAP1 [GST-tagged]

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Species Reactivity: human  

Source: E.coli  

Formula: 50 mM Tris/HCl pH 7.5, 0.1 mM EGTA, 150 mM NaCl, 0.1% ß-Mercaptoethanol,270 mM Sucrose, 0.03% Brij-35  

Concentration: 0.18 mg/ml  

Molecular Weight: ~107 kDa  

Protein Sequence: Accession number: AAH01596. For full protein sequence information download the Certificate of Analysis pdf.   

Quality Control Protein Identification: Confirmed by mass spectrometry  

Quality Control Activity: The activity of GST-BAP1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-BAP1 was compared confirming the deubiquitylating activity of GST-BAP1.