![LC3b [GST-tagged]](https://cdn11.bigcommerce.com/s-29gjipdr4d/images/stencil/608x608/products/9911/10244/vendor-image-ubiquigent__10304.1620921728__94715.1634138980.jpg?c=1 "LC3b [GST-tagged]")
![LC3b [GST-tagged]](https://cdn11.bigcommerce.com/s-29gjipdr4d/images/stencil/1280x1280/products/9911/10244/vendor-image-ubiquigent__10304.1620921728__94715.1634138980.jpg?c=1?imbypass=on "LC3b [GST-tagged]")
Description
Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.
Species Reactivity: human
Source: E.coli
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Concentration: 1 mg/ml
Molecular Weight: ~41.48 kDa
Protein Sequence: Accession number: NP_073729.1. For full protein sequence information download the Certificate of Analysis pdf.
Quality Control Protein Identification: Confirmed by mass spectrometry.
Quality Control Activity: E1 Thioester LC3b Loading Assay: The activity of LC3b was validated by loading LC3b onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of LC3b and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the LC3b/His-ATG7 thioester bond to the reducing agent DTT was confirmed.
