LC3b [GST-tagged]

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Species Reactivity: human  

Source: E.coli  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~41.48 kDa  

Protein Sequence: Accession number: NP_073729.1. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E1 Thioester LC3b Loading Assay: The activity of LC3b was validated by loading LC3b onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of LC3b and ATP at 30 °C was compared at two time points, T0 and T10 minutes.   Sensitivity of the LC3b/His-ATG7 thioester bond to the reducing agent DTT was confirmed.