UBE2J2 (NCUBE2) residues (1-226) [6His-tagged]

Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.

Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~29 kDa  

Protein Sequence: Accession number: NP_477515.2. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity:  E2-Ubiquitin Thioester Loading Assay: The activity of His-UBE2J2 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the His-UBE2J2 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and His-UBE2J2 enzymes in the presence of ubiquitin and ATP at 30 °C was compared at two time points, T0 and T10  minutes. Sensitivity of the ubiquitin/His-UBE2J2 thioester bond to the reducing agent DTT was confirmed.