![UBE2J2 (NCUBE2) residues (1-226) [untagged]](https://cdn11.bigcommerce.com/s-29gjipdr4d/images/stencil/608x608/products/9993/10326/vendor-image-ubiquigent__43978.1620921758__33550.1634139008.jpg?c=1 "UBE2J2 (NCUBE2) residues (1-226) [untagged]")
![UBE2J2 (NCUBE2) residues (1-226) [untagged]](https://cdn11.bigcommerce.com/s-29gjipdr4d/images/stencil/1280x1280/products/9993/10326/vendor-image-ubiquigent__43978.1620921758__33550.1634139008.jpg?c=1?imbypass=on "UBE2J2 (NCUBE2) residues (1-226) [untagged]")
Description
Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.
Species Reactivity: Human
Source: E. coli expression
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Concentration: 1 mg/ml
Molecular Weight: ~27 kDa
Protein Sequence: Accession number: NP_477515.2. For full protein sequence information download the Certificate of Analysis pdf.
Quality Control Protein Identification: Confirmed by mass spectrometry.
Quality Control Activity: E2-Ubiquitin Thioester Loading Assay: The activity of UBE2J2 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the UBE2J2 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and UBE2J2 enzymes in the presence of ubiquitin and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/UBE2J2 thioester bond to the reducing agent DTT was confirmed.
