Ubiquitin-Rhodamine 110

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Species Reactivity: human  

Source: synthetic  

Formula: DMSO  

Concentration: 2mg/ml  

Molecular Weight: 8.93kDa  

Protein Sequence: Accession number: P62987. C-terminally tagged with Rhodamine 110  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: Activity Assay:  The activity of Ubiquitin-Rhodamine 110 was validated by  determining the increase in fluorescence at 535nm (Excitation  485nm) measured as a result of the enzyme catalysed  cleavage at the amide bond between the C-terminal  Glycine and Rhodamine, generating Ubiquitin and de-  quenched Rhodamine 110-Glycine. UCHL3 (deubiquity-lase) was incubated with Ubiquitin-Rhodamine 110 and  the fluorescence was measured at four time points (0min,  30min, 60min and 90min).