Description
Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.
Species Reactivity: human
Source: synthetic
Formula: DMSO
Concentration: 2mg/ml
Molecular Weight: 8.72kDa
Protein Sequence: Accession number: P62987. C-terminally tagged with AMC
Quality Control Protein Identification: Confirmed by mass spectrometry.
Quality Control Activity: Activity Assay: The activity of Ubiquitin-AMC (7-amido-4-methylcoumarin) was validated by determining the increase in fluorescence at 460nm (Excitation 380nm). Increased fluorescence is a result of the enzyme catalysed cleavage between the C-terminal Glycine and AMC, creating Ubiquitin and de-quenched AMC. UCHL3 (deubiquitylase) was incubated with Ubiquitin-AMC and the fluorescence was measured at four time points (0min, 30min, 60min and 90min).
