Description
- SKU:
- B-1185-2
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ] , Thorn apple/ Jimson weed Lectin
- Application:
- Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT, FLUOROSPOT
- Conjugate:
- Biotin
- Extra Details:
- DSL contains two chains of 40kDa and 46kDa joined by disulfide bonds. This lectin is free of a reported 32kDa contaminant protein. The carbohydrate binding site recognizes (?-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (?-1,6) and (?-1,2) was reported to be the most potent inhibitor of agglutination. Biotinylated Datura stramonium lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: Chitin Hydrolysate
- Purification:
- Affinity Purified
- Research Area:
- Glycobiology
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8 [o]C; Store frozen for long term storage
- Supplier:
- Vector Laboratories
- Size:
- 2mg
- Manufacturer's Data Sheet:biotinylated-datura-stramonium-lectin-dsl.html
