DCNL1 [GST-tagged]

Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.

Species Reactivity: human  

Source: E. coli  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: 57.1kDa  

Protein Sequence: Accession number: NP_65691.2 For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E3 Ligase Assay: The activity of GST-DCNL1 was validated through its ability to enhance the neddylation of Cul1/Rbx1/Skp1 acting as a substrate in the presence of the thioester-loaded His-Ube2M~NEDD8. Incubation of Cul1/Rbx1/Skp1 and thioester loaded His-Ube2M~NEDD8 in the presence or absence of GST-DCNL1 at 4 °C was compared at two time points T0 and T2 minutes. Increased neddylation of the Cul1 subunit in the presence of GST-DCNL1 was demonstrated.