![DCNL1 [GST-tagged]](https://cdn11.bigcommerce.com/s-29gjipdr4d/images/stencil/608x608/products/10052/10385/vendor-image-ubiquigent__17878.1620921777__28026.1634139029.jpg?c=1 "DCNL1 [GST-tagged]")
![DCNL1 [GST-tagged]](https://cdn11.bigcommerce.com/s-29gjipdr4d/images/stencil/1280x1280/products/10052/10385/vendor-image-ubiquigent__17878.1620921777__28026.1634139029.jpg?c=1?imbypass=on "DCNL1 [GST-tagged]")
Description
Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.
Species Reactivity: human
Source: E. coli
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Concentration: 0.5 mg/ml
Molecular Weight: 57.1kDa
Protein Sequence: Accession number: NP_65691.2 For full protein sequence information download the Certificate of Analysis pdf.
Quality Control Protein Identification: Confirmed by mass spectrometry.
Quality Control Activity: E3 Ligase Assay: The activity of GST-DCNL1 was validated through its ability to enhance the neddylation of Cul1/Rbx1/Skp1 acting as a substrate in the presence of the thioester-loaded His-Ube2M~NEDD8. Incubation of Cul1/Rbx1/Skp1 and thioester loaded His-Ube2M~NEDD8 in the presence or absence of GST-DCNL1 at 4 °C was compared at two time points T0 and T2 minutes. Increased neddylation of the Cul1 subunit in the presence of GST-DCNL1 was demonstrated.
