Periodic Acid-Schidd (PAS) -Mayer's Hematoxylin

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Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Mayer’s hematoxylin is used to stain cell nuclei.

Uses and sample preparation:
Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

Components:


• Schiff Reagent

• Periodic acid 0,5%

• Sulfurose Acid Reagent

• Mayer’s Hematoxylin


Short Procedure:


1. Deparaffinize and hydrate

2. Add periodic acid 0,5%

3. Rinse with distilled water

4. Add Schiff Reagent

5. Rinse with 3 baths of sulfurose acid

6. Rinse with tap water

7. Place in hematoxylin

8. Rinse with tap water

9. Dehydrate, clear and coverslip


Staining characteristics:
Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polisaccharides, glicolipids and basement membrane in red/purple.