Description
Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.
Species Reactivity: Human
Source: E. coli expression
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Concentration: 1 mg/ml
Molecular Weight: ~23 kDa
Protein Sequence: Accession number: AAH09139. For full protein sequence information download the Certificate of Analysis pdf.
Quality Control Protein Identification: Confirmed by mass spectrometry.
Quality Control Activity: E2-Ubiquitin Thioester Loading Assay: The activity of UBE2E1 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the UBE2E1 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and UBE2E1 enzymes in the presence of ubiquitin and ATP at 30 ðC was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/UBE2E1 thioester bond to the reducing agent DTT was confirmed.